HPLC Analysis of Insecticide Methiocarb on Coresep 100 Mixed-Mode Column
Application description
Methiocarb is carbamate pesticide used as a bird repellent, insecticide, and acaricide. This and other carbamates are widely used in agriculture. Methiocarb is hydrophobic and neutral in nature. It was analyzed on Coresep 100 in reversed-phase mode. The compound is well retained with perfect peak shape. This robust method can be used for analysis of various pesticides. The method is fully compatible with mass spectrometry.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Herbicide, Pesticide, Pyridine |
Nature of compounds: | Basic, Hydrophobic |
Compounds: | Dursban |