HPLC Analysis of Insecticide Parathion-Ethyl on Coresep 100 Mixed-Mode Column
Application description
Parathion-ethyl is organophosphate insecticide and acaricide. It is highly toxic to humans with strict limits. We have developed a method for analysis of parathion on Coresep 100 mixed-mode HPLC column. Parathion is well retained with perfect peak shape. The method can be used for analysis of various contaminants in different matrices. Column and mobile phase are compatible with all major detection techniques including mass spectrometry (LC/MS).
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Insecticide, Pesticide |
Nature of compounds: | Hydrophobic, Neutral |
Compounds: | Parathion-Ethyl |