HPLC Analysis of Insecticide Parathion-Ethyl on Coresep 100 Mixed-Mode Column

Application description

Parathion-ethyl is organophosphate insecticide and acaricide. It is highly toxic to humans with strict limits. We have developed a method for analysis of parathion on Coresep 100 mixed-mode HPLC column. Parathion is well retained with perfect peak shape. The method can be used for analysis of various contaminants in different matrices. Column and mobile phase are compatible with all major detection techniques including mass spectrometry (LC/MS).

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Insecticide, Pesticide
Nature of compounds: Hydrophobic, Neutral
Compounds: Parathion-Ethyl