HPLC Analysis of Insecticide Profenofos and Related Impurities on Coresep 100 Mixed-Mode Column

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Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 30% to 70% , in 7 min, 3 min hold, 20 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Aromatic acid, Aromatic base, Drug, Insecticide, Pesticide
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Profenofos, Related Impurities