HPLC Analysis of Insecticide Propoxur on Coresep 100 Mixed-Mode Column

Application description

Propoxur is carbamate insecticide. It is used against turf, forestry, household pests, and fleas. Propoxur is toxic to birds, fish, and aquatic species. Propoxur is hydrophobic and neutral in nature. It is retained on Coresep 100 mixed-mode column by reversed-phase mechanism. Retention time is controlled by the amount of ACN in the mobile phase. This method is robust and reproducible and can be used for analysis of other agricultural products. The method is LC/MS compatible with good retention and peak shape for Propoxur. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Herbicide, Insecticide
Nature of compounds: Hydrophobic, Neutral
Compounds: Propoxur