HPLC Analysis of Isomers of Toluidine on Core-Shell Mixed-Mode Coresep 100 Column

Application description

Toluidines are aromatic amines used as building blocks for the synthesis of various chemicals. Toluidine has been used in preparation of aromatic azo compounds and bidentate Schiff base ligands, via condensation with salicylaldehyde. The chemical properties of the Toluidines are quite similar to those of aniline, and toluidines have properties in common with other aromatic amines. Due to the amino group bonded to the aromatic ring, the toluidines are weakly basic compounds. There are three isomers of Toluidine which are very close in chemical properties in terms of hydrophobicity and basicity (pKa value of Toluidines is 4.4-5.1). All three isomers were analyzed and separated on the core-shell mixed-mode reversed-phase cation-exchange Coresep 100 column. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, and can be used for analysis of isomers of Toluidines in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with the Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. The method does not require ion-pairing reagents. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: MeCN 50% with 30 mM AmFm pH 3.2
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Amines, Aromatic base, Isomer
Nature of compounds: Basic, Hydrophobic, Polar
Compounds: o-Toluidine, m-Toluidine, p-Toluidine