HPLC Analysis of Medication Guaifenesin and Related Impurities on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: A: 15% ACN with 0.02% H2SO4
B: 50% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold
Detection: UV 255
Sample: 2 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Guaifenesin, Related Impurities