Application description
Guaifenesin is medication which is used to help with clearing airways from phlegm and mucus. It is a relatively hydrophobic and neutral compound. We have developed a method for analysis of guaifenesin and related impurities in various drug formulations. The compound is retained on a Coresep 100 column by a pure reversed-phase mechanism. Retention time is controlled by the amount of acetonitrile in the mobile phase. Buffer selection depends on the detection technique used to monitor guaifenesin and related impurities. Coresep 100 HPLC column is a reversed-phase cation-exchange column, based on core-shell particles. It demonstrates very high efficiency and robustness as well as a unique alternative to RP and HILIC selectivity.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase, cation-exchange |
| Column Dimenstions: |
4.6x150 mm, 2.7 um, 90A |
| Mobile Phase: |
A: 15% ACN with 0.02% H2SO4
B: 50% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold |
| Detection: |
UV 255 |
| Sample: |
2 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic base, Aromatic compound, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
| Compounds: |
Guaifenesin, Related Impurities |
