HPLC Analysis of Melatonin on Heritage MA Mixed-Mode Column

HPLC Analysis of Melatonin on Heritage MA Mixed-Mode Column chromatogram
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Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase, cation-exclusion
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 25% ACN with 30 mM AmAc pH 4
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Melatonin