HPLC Analysis of Mono-, Di- and Tricarboxylic Acids on Amaze HA Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | ACN/water/phosphoric acid and ACN/water/ammonium phosphate buffer |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Organic acid, Preservative |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Citric acid, Fumaric acid, Oxalic acid, Maleic acid, Acetic acid, Formic acid |