HPLC Analysis of Mono-, Di- and Tricarboxylic Acids on Amaze HA Mixed-Mode Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: reveresed-phase , anion-exchange
Column Dimenstions: 4.6x100, 3 um, 100A
Mobile Phase: ACN/water/phosphoric acid and ACN/water/ammonium phosphate buffer
Detection: 205 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid, Preservative
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Citric acid, Fumaric acid, Oxalic acid, Maleic acid, Acetic acid, Formic acid