HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column

Application description

Parabens are used as preservatives in food and drugs. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions. Parabens are neutral and hydrophobic in nature, and they are retained on a Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component of the mobile phase. This method is robust and reproducible.

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 10 mM Am Ac ph 4
Detection: UV 255 nm
Sample: 0.2 mg/ml
Injection: 3 ul
Flow rate: 1 ml/min
Class of compounds: Aromatic compound, Organic compound, Preservative
Nature of compounds: Hydrophobic, Neutral
Compounds: Methylparaben, Ethylparaben, Propylparaben