Application description
Parabens are used as preservatives in food and drugs. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions. Parabens are neutral and hydrophobic in nature, and they are retained on a Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component of the mobile phase. This method is robust and reproducible.
Conditions of Experiment
| Column: |
Heritage MA |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
4.6x150 mm, 3 um, 100A |
| Mobile Phase: |
60% ACN with 10 mM Am Ac ph 4 |
| Detection: |
UV 255 nm |
| Sample: |
0.2 mg/ml |
| Injection: |
3 ul |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic compound, Organic compound, Preservative |
| Nature of compounds: |
Hydrophobic, Neutral |
| Compounds: |
Methylparaben, Ethylparaben, Propylparaben |
