HPLC Analysis of Pesticide Dursban on Coresep 100 Mixed-Mode Column

Application description

Dursban or Chlorpyrifos (CPS) is an organophosphate pesticide. It is used in crops, animals, and buildings. CPS is moderately hazardous to humans. Dursban is hydrophobic and basic in nature. It is retained on Coresep 100 mixed-mode column by reversed-phase and cation-exchange mechanism. Chlorpyrifos is well retained with perfect peak shape. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. This method is compatible with LC/Ms and does not require ion-pairing reagent. The column and method can be used for analysis of multiple pesticides, herbicides, insecticides, and other agricultural products.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Aromatic compound, Fungicide
Nature of compounds: Hydrophobic, Neutral
Compounds: Tebuconazole