HPLC Analysis of Pesticide pCPA on Heritage MA Mixed-Mode Column

Application description

4-Chlorophenoxyacetic acid or parachlorophenoxyacetate (pCPA) is a synthetic pesticide similar to chemicals in a group of plant hormone called auxins. 4-chlorophenoxy acetic acid (4-CPA), a chlorine derivative of phenoxyacetic acid (PA), is a plant growth regulator used as a herbicide. pCPA is hydrophobic and acidic in nature. It has carboxylic acid fragment with pKa of 3.56. pCPA was analyzed on Heritage mixed-mode column in reversed-phase anion-exchange mode. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6 x 50 mm
Mobile Phase: 60% ACN with 80 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Class of compounds: Organic acid, Pesticide
Nature of compounds: Acidic, Hydrophobic
Compounds: pCPA (4-Chlorophenoxyacetic Acid)