HPLC Analysis of Piperazine on Amaze HD Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: reveresed-phase, cation-exchanage
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 20% ACN with 0.2% TFA
Detection: ELSD, 40*C
Sample: 2.5 mg/ml
Injection: 1 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Organic compound
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Piperazine