HPLC Analysis of Plant Growth Regulator Diclobutrazol and Related Impurities on Coresep 100 Mixed-Mode Column

HPLC Analysis of Plant Growth Regulator Diclobutrazol and Related Impurities on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: 40% ACN with 50 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Diclobutrazol
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Drug, Fungicide, Herbicide
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral

Application description

Diclobutrazol is a racemic mixture of two chemical compounds from the triazole group, which acts as a fungicide. Itis normally applied to the soil to be taken up by the roots and transported via the xylem to the upper parts of the plant. Seeds can be soaked with diclobutrazol to reduce seedling growth. Diclobutrazol is hydrophobic and slightly basic. It is retained on Coresep 100 column by combining a strong reversed-phase mechanism with a weak cation-exchange mechanism. Retention time is controlled by amount of ACN and to a lesser degree by buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of diclobutrazol in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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