HPLC Analysis of Preservative Propylparaben on Heritage C18 Column According to US Pharmacopeia

Application description

United States Pharmacopeia establishes written procedures for analysis of drugs, food ingredients, dietary supplement products and other ingredients. These standard procedures are used by pharmaceutical, chemical and other companies to ensure identity and purity of various compounds. Propylparaben, along with methylparaben and ethylparaben, is a preservative used in water-based cosmetics and food. Parabens are hydrophobic neutral compounds that can be easily retained on a Heritage C18 HPLC column. Analysis and column are robust and reproducible and can be used in determination of parabens in various samples. Retention time is controlled by amount of ACN or MeOH in the mobile phase. Buffers and additives can be chosen to accommodate different detection techniques.

Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 50% MeCN with 20 mM NaH2PO4 pH 2.5
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic compound, Preservative
Nature of compounds: Hydrophobic, Neutral
Compounds: Propylparaben