HPLC Analysis of Pseudoephedrine and Citric Acid on Heritage N Column

Conditions of Experiment
Column: Heritage N
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 60% ACN with 20 mM Na2HPO4 pH 7
Detection: UV 205 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Drug, Organic acid, Preservative, Stimulant
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Citric acid, Pseudoephedrine