HPLC Analysis of Pyridoxal, Pyridoxine, Pyridoxal 5-’Phosphate and Doxylamine on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: 80% ACN with 30 mM AmFm pH 3.5
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Drug, Vitamin
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Doxylamine, Pyridoxal, Pyridoxine, Pyridoxal 5′-phosphate