HPLC Analysis of Robittusin Severe Cold & Sore Throat Formulation on Coresep 100 Mixed-Mode Column

Application description

Robitussin Severe Cold & Sore is a drug combination consisting of active ingredients and excipients. Active ingredients include acetaminophen and dextromethorphan. Excipients or inactive ingredients include anhydrous citric acid, edetate disodium, FD&C red no. 40, glycerin, menthol, natural and artificial flavors, polyethylene glycol, propyl gallate, propylene glycol, purified water, sodium benzoate, sodium citrate, sorbitol solution, sucralose, triacetin, xanthan gum. Complex composition like this can be successfully analyzed in isocratic or gradient mode on Coresep 100 mixed-mode core-shell reversed-phase cation-exchange column. Compounds are retained and separated by reversed-phase, cation-exchange and anion-exclusion mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is robust and reproducible and can be used for analysis of other over-the-counter (OTC) formulations. Depending on the properties of the compounds and detection technique, other buffers and additives can be used to achieve the desired separation, selectivity, and resolution between critical compounds. Compounds are well retained without the use of ion-pairing reagent. The column is compatible with 100% water and 100% ACN. Other drug formulation can be analyzed on mixed-mode columns with perfect peak shape, retention control, and robustness. Coresep 100 columns can be used with any of the common detection techniques (UV, ELSD, RI, mass-spec, CAD). Mixed-mode HPLC columns provide 2-D selectivity for the retention of hydrophobic and hydrophilic ionizable compounds.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: "A: 10% ACN with 0.02% H2SO4
B: 70% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold "
Detection: UV 275
Sample: various
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral
Compounds: Acetaminophen, Dextromethorphan