HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-Exchange Modes

Conditions of Experiment
Column: Amaze TR
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x50 mm, 3 um, 100A
Mobile Phase: A - 75% ACN with 5 mM AmFm pf 3
B - 20% ACN with 20 mM AmFm pH 2.1
from 100% A to 100% B in 4 min, 2 min hold
Detection: ELSD, MS
Sample: 0.5-0.8 mg/ml each
Injection: 3 uL
Flow rate: 0.7 ml/min
Analytes
Class of compounds: Amines, Polyamine, Polymer
Nature of compounds: Basic, Hydrophilic
Compounds: Putrescene, Spermidine, Spermine