Home HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-Exchange Modes
Conditions of Experiment
| Column: |
Amaze TR |
| Separation Modes: |
HILIC, cation-exchange |
| Column Dimenstions: |
3.0x50 mm, 3 um, 100A |
| Mobile Phase: |
A - 75% ACN with 5 mM AmFm pf 3
B - 20% ACN with 20 mM AmFm pH 2.1
from 100% A to 100% B in 4 min, 2 min hold |
| Detection: |
ELSD, MS |
| Sample: |
0.5-0.8 mg/ml each |
| Injection: |
3 uL |
| Flow rate: |
0.7 ml/min |
Analytes
| Class of compounds: |
Amines, Polyamine, Polymer |
| Nature of compounds: |
Basic, Hydrophilic |
| Compounds: |
Putrescence, Spermidine, Spermine |
