HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode Column

Conditions of Experiment
Column: Amaze TR
Separation Modes: reversed-phase, ction-exchange, cation-exlusion
Column Dimenstions: 3.0x50 mm, 3 um, 100A
Mobile Phase: A - 0% ACN with 2 mM AmFm pf 3.8
B - 15% ACN with 15 mM AmFm pH 2.4
from 100% A to 100% B in 6 min
Detection: ELSD, MS
Sample: 0.5-0.8 mg/ml each
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Polyamine, Polymer
Nature of compounds: Basic, Hydrophilic
Compounds: Putrescence, Spermidine, Spermine, N1-Acetylspermidine