Home HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode Column
Conditions of Experiment
| Column: |
Amaze TR |
| Separation Modes: |
reversed-phase, ction-exchange, cation-exlusion |
| Column Dimenstions: |
3.0x50 mm, 3 um, 100A |
| Mobile Phase: |
A - 0% ACN with 2 mM AmFm pf 3.8
B - 15% ACN with 15 mM AmFm pH 2.4
from 100% A to 100% B in 6 min |
| Detection: |
ELSD, MS |
| Sample: |
0.5-0.8 mg/ml each |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Amines, Polyamine, Polymer |
| Nature of compounds: |
Basic, Hydrophilic |
| Compounds: |
Putrescene, Spermidine, Spermine, N1-Acetylspermine |
