HPLC Analysis of Substituted Pyridines on Amaze SC Mixed-Mode Column

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 5% ACN with 0.1% phosphoric acid
Detection: UV 255 nm
Sample: 0.2-0.3 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Aromatic base, Aromatic compound, Isomer, Pyridine, Vitamin
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: 2-Pyridinecarboxylic acid, 3-Pyridinecarboxylic acid, 4-Pyridinecarboxylic acid, Nicotinamide, Pyridoxal, Pyridoxine