HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 5
Detection: ELSD, 40°C
Sample: 2 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Inorganic anion, Sugar, Supplement
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Chloride Ion, Sucrose, Histidine, Arginine