HPLC Analysis of Sugar Alcohols and Sugars on Amaze HD Mixed-Mode Column

Application description

Sugar alcohols and sugars are carbohydrates which are used in food and can be found in fruits, vegetables and other naturally occurring products. Simple sugars include monosaccharides like glucose, fructose and galactose. Sugar alcohols are derived from sugars and are occurring naturally, or synthesized by hydrogenation of sugars. Both sugars and sugar alcohols are polar with no chromophores. The most common approach for the analysis of sugars and sugar alcohols is HILIC chromatography. These hydrophilic compounds are retained by a polar mechanism on a polar stationary phase like Amaze HD. This column has a very polar group on the surface of silica gel which allows the use of less ACN for the retention of sugars and sugar alcohols. This method and the column can be used for the analysis of sugars and sugar alcohols in fruits, veggies, juices and other beverages. The Amaze HD is compatible with 100% ACN and 100% water. ELSD, CAD, RI or MS are required to retain and monitor sugars.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD 40°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Xylitol, Mannitol, Sucrose, Maltose, Melezitose