HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: ACN/Formic acid – 95/5
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Maltitol, Sucrose, Lactose