HPLC Analysis of THC-Infused Black Tea

Application description

Psychoactive cannabinoids like tetrahydrocannabinol are used in medical and recreational cannabis-based edibles and beverages. Tetrahydrocannabinol is neutral and very hydrophobic in nature. We have developed an approach for the analysis of cannabis related products on the Daze 47 HPLC column. The column and method can be used for the analysis of main cannabinoids in flowers, plants, edibles, beverages, patches, creams etc. Some sample preparation might be required for more complex matrices like cookies, brownies and other baked and cooked goods. The detection is done by UV. Our method is robust, short and efficient and can be used for the analysis of tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabinolic acid (CBNA), cannabigerol (CBG), cannabichromene (CBC), cannabivarin (CBV), cannabicyclol (CBL), tetrahydrocannabivarin (THCV) cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabieeeelsoin (CBE) and cannabicitran (CBT) in cannabinoid-related products (flowers, extracts, cannabis infused edibles and drinks, creams and patches).

Conditions of Experiment
Column: Daze 47
Separation Modes:
Column Dimenstions: 3.0 x 150 mm, 5 um, 100A
Mobile Phase: 70% ACN with 0.1% H2SO4
Detection: UV 275 nm
Flow rate: 1.0 mL/min
Class of compounds: Aromatic compound, Cannabinoid, Controlled substance
Nature of compounds: Hydrophobic, Neutral
Compounds: THC