Application description
Psychoactive cannabinoids like tetrahydrocannabinol are used in medical and recreational cannabis-based edibles and beverages. Tetrahydrocannabinol is neutral and very hydrophobic in nature. We have developed an approach for the analysis of cannabis related products on the Daze 47 HPLC column. The column and method can be used for the analysis of main cannabinoids in flowers, plants, edibles, beverages, patches, creams etc. Some sample preparation might be required for more complex matrices like cookies, brownies and other baked and cooked goods. The detection is done by UV. Our method is robust, short and efficient and can be used for the analysis of tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabinolic acid (CBNA), cannabigerol (CBG), cannabichromene (CBC), cannabivarin (CBV), cannabicyclol (CBL), tetrahydrocannabivarin (THCV) cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabieeeelsoin (CBE) and cannabicitran (CBT) in cannabinoid-related products (flowers, extracts, cannabis infused edibles and drinks, creams and patches).
Conditions of Experiment
Column: |
Daze 47 |
Separation Modes: |
|
Column Dimenstions: |
3.0 x 150 mm, 5 um, 100A |
Mobile Phase: |
70% ACN with 0.1% H2SO4 |
Detection: |
UV 275 nm |
Sample: |
|
Injection: |
|
Flow rate: |
1.0 mL/min |
Analytes
Class of compounds: |
Aromatic compound, Cannabinoid, Controlled substance |
Nature of compounds: |
Hydrophobic, Neutral |
Compounds: |
THC |