HPLC Analysis of Three Cannabinoids on Cores-Shell Mixed-Mode Coresep 100 Column

Application description

Three main cannabinoids (THC, CBD and CBN) were analyzed on a Coresep 100 core-shell mixed-mode column. Tetrahydrocannabinol is a psychoactive component of cannabis.THC is a lipid. The precursor of THC is tetrahydrocannabinolic acid (THCA). Cannabidiol is a major phytocannabinoid. CBD does not have any psychoactive properties, but constitutes up to 40% of plants extracts. Cannabinols are non-psychoactive cannabinoids which occurs in trace amounts in marijuana plants. All three compounds are hydrophobic and neutral, and are separated by reversed-phase mechanism. Method can be used in analysis of active components of various marijuana related products (flowers, buds, edibles, infused products, etc.). HPLC method is reproducible and robust.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 0.1% of phosphoric acid
Detection: UV 210 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1.5 ml/min
Class of compounds: Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: CBD, CBN, THC