Application description
Three main cannabinoids (THC, CBD and CBN) were analyzed on a Coresep 100 core-shell mixed-mode column. Tetrahydrocannabinol is a psychoactive component of cannabis.THC is a lipid. The precursor of THC is tetrahydrocannabinolic acid (THCA). Cannabidiol is a major phytocannabinoid. CBD does not have any psychoactive properties, but constitutes up to 40% of plants extracts. Cannabinols are non-psychoactive cannabinoids which occurs in trace amounts in marijuana plants. All three compounds are hydrophobic and neutral, and are separated by reversed-phase mechanism. Method can be used in analysis of active components of various marijuana related products (flowers, buds, edibles, infused products, etc.). HPLC method is reproducible and robust.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
50% ACN with 0.1% of phosphoric acid |
| Detection: |
UV 210 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1.5 ml/min |
Analytes
| Class of compounds: |
Aromatic compound, Cannabinoid, Controlled substance, Drug |
| Nature of compounds: |
Hydrophobic, Neutral |
| Compounds: |
CBD, CBN, THC |
