HPLC Analysis of Toluidine Isomers on Core-Shell Mixed-Mode Coresep S Column in Cation-Exchange Mode

Application description

Isomers of toluidine were separated on the core-shell HILIC/mixed-mode column based on their ionic properties. Only ion-exchange mechanisms are involved in this particular application, which proves that mixed-mode columns can be used in a single-mode or a mixed-mode with the same great success.

Conditions of Experiment
Column: Coresep S
Separation Modes: cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 5% of ACN with 25 mM AmAc pH 5
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1-2 ml/min
Class of compounds: Amines, Aromatic base, Isomer
Nature of compounds: Basic, Hydrophobic, Polar
Compounds: o-Toluidine, m-Toluidine, p-Toluidine