Application description
Isomers of toluidine were separated on the core-shell HILIC/mixed-mode column based on their ionic properties. Only ion-exchange mechanisms are involved in this particular application, which proves that mixed-mode columns can be used in a single-mode or a mixed-mode with the same great success.
Conditions of Experiment
| Column: |
Coresep S |
| Separation Modes: |
cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
5% of ACN with 25 mM AmAc pH 5 |
| Detection: |
UV 250 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1-2 ml/min |
Analytes
| Class of compounds: |
Amines, Aromatic base, Isomer |
| Nature of compounds: |
Basic, Hydrophobic, Polar |
| Compounds: |
o-Toluidine, m-Toluidine, p-Toluidine |
