HPLC Analysis of Vanillylmandelic Acid and Related Impurities on Coresep SB Mixed-Mode Column

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 40% ACN with 50 mM AmFm pH 3
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Aromatic base, Catecholamine, Drug, Neurotransmitter
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Vanillylmandelic Acid, Related Impurities