HPLC Analysis of Vanillylmandelic Acid and Related Impurities on Coresep SB Mixed-Mode Column

HPLC Analysis of Vanillylmandelic Acid and Related Impurities on Coresep SB Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep SB
Separation Modes:
reversed-phase and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 40% ACN with 50 mM AmFm pH 3
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Vanillylmandelic Acid
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Catecholamine, Drug, Neurotransmitter
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar

Application description

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