HPLC Analysis of Vick’s NyQuil Cold and Flu Formulation on Coresep 100 Mixed-Mode Column

Application description

Vick’s NyQuil Cold and Cough remedy temporarily relieves common cold/flu symptoms: cough due to minor throat & bronchial irritation, sore throat, headache, minor aches & pains - fever - runny nose & sneezing. The active ingredients include acetaminophen, chlorpheniramine, and dextromethorphan. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Chlorpheniramine hydrophobic and basic antihistamine drugs are used to treat symptoms of allergic conditions. All three active ingredients retain very differently, and usually, dextromethorphan and chlorpheniramine show a poor peak shape in RP chromatography due to residual silanol interactions. This problem does not exist on Coresep 100 mixed-mode column, where the polar ionizable group shields analytes from interacting with the residual silanols. Coresep 100 column demonstrates high efficiency and selectivity of separation. Methods for this drug composition are robust and reproducible and can be used in method development and validation. The column is compatible with 100% ACN and 100% water and can be used in a single or dual mode depending on the nature of the analyte and mobile phase composition. Very often order of elution can be changed by changing the amount of ACN, buffer pH, buffer concentration, and buffer nature.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 0.02% H2SO4
B: 70% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold
Detection: UV 275
Sample: various
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral
Compounds: Acetaminophen, Chlorpheniramine, Dextromethorphan