HPLC Analysis of Vildagliptin on Amaze SC Mixed-Mode Column. Effect of Acetonitrile and Acid Concentrations

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/Water/H2SO4
Detection: 210 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Drug
Nature of compounds: Hydrophobic
Compounds: Vildagliptin