HPLC Analysis of Wal-Tussin DM MAX Cough & Chest Congestion Formulation on Coresep 100 Mixed-Mode Column

Application description

Cough, cold and flu medications are a complex mixture of active ingredients and excipients. It usually contains hydrophilic and hydrophobic drugs, counterions, other organic and inorganic additives. Analysis of such complex mixtures are a challenging task. Analysis is usually done with several methods, including reversed-phase and ion-exchange chromatography. Active ingredients of Wal-Tussin DM MAX (guaifenesin and dextromethorphan and excipients were separated on Coresep 100 mixed-mode reversed-phase and cation-exchange HPLC column. Guaifenesin is a hydrophilic neutral compound which is added to cough and cold formulations to help with coughing thick mucus it is used in combination with dextromethorphan to alleviate symptoms related to cold, flu and congestion. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Guaifenesin is retained by a pure reversed-phase mechanism, and dextromethorphan is retained by a combination of reversed-phase and cations-exchange mechanisms. This HPLC method demonstrates baseline separation and robustness in analysis of cough and cold formulations. Coresep 100 columns can be successfully used for analysis of various compounds, drug compositions and formulations. The column is compatible with 100% water and 100% ACN and can be used in isocratic or various gradient modes. The retention of compounds is adjusted by changing the amount of ACN, buffer pH, and buffer concentration. The method can be applied to the analysis of drugs in biofluids. Most common detection techniques can be used (UV, ELSD, mass-spec, etc.). Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 0.02% H2SO4
B: 70% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold
Detection: UV 275
Sample: various
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Guaifenesin, Dextromethorphan