HPLC Analysis of Xylitol, Sorbitol and Sucrose on Amaze HD Mixed-Mode Column

Application description

Sugars are polar non-ionic compounds. They are usually analyzed on HILIC columns. We have developed a new column and method for analysis of mono, di- and other saccharides by HPLC. Sugars are retained by a pure HILIC mechanism. The polarity of the stationary phase can be changed based on the pH of the mobile phase and buffer concentrations. The presence of salt is desired to increase the retention time of sugars. In general, monosaccharides will retain less tan di and trisaccharides. The Amaze HD column is compatible with 100% ACN and with 100% water, and can be used either in isocratic or wide gradient modes. This method is robust, reproducible, and allows the analyzation of all polar sugars and other polar compounds on one column.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD 40°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Xylitol, Sorbitol, Sucrose