HPLC Analysis of Yellow 5 (Tartrazine) Dye in Soft Drinks on Amaze TH Mixed-Mode Column

Application description

Tartrazine (Yellow 5) is a synthetic yellow azo dye used as a food additive. It is used to provide yellow color to drinks, drug formulations and various foods. Tartrazine is used as a component of various personal care products, household cleaning products, crayons, face paint, etc. It is also known as Yellow 5, E-102, FD&C Yellow 5, Acid Yellow 23 and trisodium 1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-5-pyrazolone-3-carboxylate). Tartrazine is a water-soluble and acidic. Like other dyes, Yellow 5 is derived from petrochemicals. The maximum absorbance for this yellow dye is at 425 nm. Tartrazine was analyzed and quantified on the Amaze TH mixed-mode column in anion-exchange mode. The approach was used to analyze tartrazine in various soft drinks. This method and the mixed-mode HPLC column can be used for analysis of other acidic dyes in various samples (drinks, food, medications, household cleaners, etc.).

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC and anion-exchnage
Column Dimenstions: 3.2 x 50 mm, 5 um, 100A
Mobile Phase: MeCN 50% with 100 mM AmFm pH 3
Detection: UV 425 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Dye
Nature of compounds: Acidic, Hydrophobic
Compounds: Tartrazine