HPLC Analysis of Zn-Dipicolylamine Complex on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase, cation-exclusion
Column Dimenstions: 4.6 x 50 mm, 5 um, 100A
Mobile Phase: gradient ACN from 10% to 80%, AmAc from 100 mM to 40 mM in 8 minutes
Detection: 275 nm
Sample: 0.2 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Pyridine
Nature of compounds: Basic, Hydrophobic
Compounds: Dipicolylamine