HPLC Analysis of Zwitterions DOTA and DO3A on Amaze SA Mixed-Mode Column

Conditions of Experiment
Column: Amaze SA
Separation Modes: reveresed-phase, cation-exchanage
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 10% ACN with 100 mM sodium phosphate pH 3
Detection: 210 nm
Sample: 2 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: DOTA, DO3A, Sodium ion