HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition
Conditions of Experiment
| Column: | Coresep SB |
| Separation Modes: | reversed-phase, anion-exchange, cation-exclusion |
| Column Dimenstions: | 4.6x150 mm, 2.7 um, 90A |
| Mobile Phase: | ACN/water/ammonium phosphate |
| Detection: | 225 nm |
| Sample: | 0.1-0.2 mg/ml |
| Injection: | 2 uL |
| Flow rate: | 1 ml/min |
Analytes
| Class of compounds: | Aromatic acid, Aromatic compound, Drug, Glycoside, Pyridine, Sugar, Supplement, Vitamin |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
| Compounds: | Acetaminophen, Ascorbic acid, Loratadine, Rutoside |