HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase, anion-exchange, cation-exclusion
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: ACN/water/ammonium phosphate
Detection: 225 nm
Sample: 0.1-0.2 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Aromatic compound, Drug, Glycoside, Pyridine, Sugar, Supplement, Vitamin
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Acetaminophen, Ascorbic acid, Loratadine, Rutoside