HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reversed-phase, anion-exchange, cation-exclusion |
Column Dimenstions: | 4.6x150 mm, 2.7 um, 90A |
Mobile Phase: | ACN/water/ammonium phosphate |
Detection: | 225 nm |
Sample: | 0.1-0.2 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Glycoside, Pyridine, Sugar, Supplement, Vitamin |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetaminophen, Ascorbic acid, Loratadine, Rutoside |