Application description
Adenosine is a purine nucleoside composed of adenine and ribose fragments. Adenine is a purine-based nucleobase. Both compounds are polar and slightly basic, with ability to form hydrogen bonding with the stationary phase. Both compounds were separated within 2 minutes with an isocratic mobile phase. Method is compatible with mass spectrometry.
Conditions of Experiment
| Column: |
Amaze HD |
| Separation Modes: |
HILIC |
| Column Dimenstions: |
3.2 x 50 mm |
| Mobile Phase: |
MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05% AmFm |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1.0 mL/min |
Analytes
| Class of compounds: |
Aromatic base, Nucleobase, Nucleoside |
| Nature of compounds: |
Basic, Hydrophilic, Polar |
| Compounds: |
Adenosine, Adenine |
