HPLC Method for Analysis of Adenosine and Adenine on Amaze HD Column

Application description

Adenosine is a purine nucleoside composed of adenine and ribose fragments. Adenine is a purine-based nucleobase. Both compounds are polar and slightly basic, with ability to form hydrogen bonding with the stationary phase. Both compounds were separated within 2 minutes with an isocratic mobile phase. Method is compatible with mass spectrometry.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05% AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Class of compounds: Aromatic base, Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Adenosine, Adenine