HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05%AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.2 mL/min
Class of compounds: Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Cytidine, Cytosine