HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column

Application description

Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm
Detection: 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Class of compounds: Nucleoside
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: 2′-Deoxyadenosine, Inosine