Application description
Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD.
Conditions of Experiment
| Column: |
Amaze HD |
| Separation Modes: |
HILIC |
| Column Dimenstions: |
3.2 x 50 mm |
| Mobile Phase: |
MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm |
| Detection: |
275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1.0 mL/min |
Analytes
| Class of compounds: |
Nucleoside |
| Nature of compounds: |
Hydrophilic, Neutral, Polar |
| Compounds: |
2′-Deoxyadenosine, Inosine |
