HPLC Method for Analysis of Nucleosides and Nucleoside Derivatives on Amaze HD Column

Application description

Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction.

Conditions of Experiment
Column: Amaze HD
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: MeCN/MeOH from 90/10 to 50/50 with 0.1% HCOOH and 0.01% AmFm in 8 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Class of compounds: Aromatic base, Nucleoside
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Thymidine, Uridine, 2′-Deoxyadenosine, Adenosine, Guanosine