Application description
Phthalic acids are aromatic dicarboxylic acids with various positions of carboxylic groups. Isomers of phthalic acid were analyzed in reversed-phase anion-exchange on Coresep SB HPLC mixed-mode column. The small difference in hydrophobicity/polarity and acidic properties of molecules contributes to great resolution between these acids. Method and HPLC column can be used for analysis of phthalic and other acids in various matrices with various detection techniques (UV, ELSD, RI, MS, etc.). Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
| Detection: |
UV 230 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.8 ml/mim |
Analytes
| Class of compounds: |
Aromatic acid, Isomer, Organic acid |
| Nature of compounds: |
Acidic, Hydrophobic |
| Compounds: |
Phthalic acid, Terephthalic acid |
