HPLC Method for Analysis of Pyrilamine, Trimipramine, Pindolol and Related Impurities on Amaze HD Column

Application description

We have developed a method for HPLC separation of three drugs - trimoramine, pyrilamine and pindolol - on an Amaze HD HPLC column. Amaze HD is Helix’s new multi-mode HPLC column that can operate in HILIC, hydrogen-bonding, normal phase, cation-exchange, and anion-exclusion modes. Pyrilamine is a first-generation antihistamine used in many over-the-counter medications. Trimipramine is a tricyclic antidepressant. Pindolol is a beta blocker used in the treatment of hypertension and angina pectoris, as well as an antidepressant. All three compounds are hydrophobic and basic in nature, and with this method, all can be resolved within 4 minutes. Method can be used for HPLC analysis of these basic hydrophobic drugs in various matrices, including biofluids, and is compatible with all detection techniques: MS, RI, UV, ELSD and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 3 um
Mobile Phase: MeCN/MeOH (70/30) with 0.5% HCOOH and 0.05% AmFm
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.5 mL/min
Class of compounds: Aromatic base, Drug, Pyridine
Nature of compounds: Basic, Hydrophobic
Compounds: Pyrilamine, Trimipramine, Pindolol