HPLC Separation of Alanine and Corresponding Esters on Amaze SC HPLC Column

HPLC Separation of Alanine and Corresponding Esters on Amaze SC HPLC Column chromatogram
Amaze SC PURCHASE LEARN ABOUT Amaze SC
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cations-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: ACN/water/phosphoric acid
Detection: 200 nm
Sample: 0.3-0.5 uL
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Aromatic acid
Nature of compounds: Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Alanine, Alanine methyl ester, Alanine ethyl ester, Alanine isopropyl ester