HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC and Anion-Exclusion Modes on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC and anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: from 85% ACN with 30 mM Ammonium Acetate pH 4
to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min
Detection: 280 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Ascorbic acid, Ascorbic acid 2-phosphate