HPLC Separation of Basic Quaternary and Acidic Herbicides in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH
Conditions of Experiment
| Column: | Amaze TCH |
| Separation Modes: | HILIC, cation-exchange, anion-exchange |
| Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
| Mobile Phase: | from 80% ACN with 5 mM ammonium formate pH 3.5 to 40% ACN with 30 mM ammonium formate pH 3.5 in 10 min |
| Detection: | Corona CAD |
| Sample: | 0.2-0.5 mg/ml |
| Injection: | 2 ul |
| Flow rate: | 1 ml/min |
Analytes
| Class of compounds: | Amines, Herbicide, Inorganic anion, Organic acid, Quaternary amine |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
| Compounds: | Chlormequat, Mepiquat, Paraquat, Diquat, Chloride Ion, Ethephon |