HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate |
Detection: | ELSD, 45*C |
Sample: | 1 mg/ml |
Injection: | 3 ul |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amines, Amino acid, Biological buffer, Inorganic cation |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | CAPS, Sodium ion, HEPES |