HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/ammonium formate
Detection: ELSD, 45*C
Sample: 1 mg/ml
Injection: 3 ul
Flow rate: 0.6 ml/min
Class of compounds: Amines, Amino acid, Biological buffer, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: CAPS, Sodium ion, HEPES