HPLC Separation of Controlled Substances on Coresep 100 Mixed-Mode Column

Application description

Morphine is an opiate which is used as pain medication. It is Schedule II controlled substance. Codeine is an opiate medication used as effective pain drug as well as a component of cough medications. Codeine is Schedule II controlled substance. Oxycodone is opioid pain medication. It is Schedule II controlled substance. Cocaine is a strong stimulant mostly used as recreational drug. It is Schedule II controlled substance. Hydrocodone is an opioid derived from codeine and one of the opioid alkaloids in opium poppy. It is a narcotic analgesic. Hydrocodone is used to treat moderate to severe pain. It is Schedule II controlled substance. Analysis of drugs of abuse and controlled substances is an important task. All 5 compounds are hydrophobic and basic in nature, thye retain on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. Amount of ACN, buffer pH and buffer concentration can be used to adjust retention time and selectivity of this separation. The method can be used for analysis of illegal drugs and controlled substances in formulations and biofluids. The method is fully compatible with mass spectrometry. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 50% to 80%, AmFm pH 3 from 20 mM to 40 mM in 10 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic base, Controlled substance, Drug, Illegal drug
Nature of compounds: Basic, Hydrophobic
Compounds: Morphine, Codeine, Oxycodone, Cocaine, Hydrocodone