HPLC Separation of Dihydrostreptomycin and Streptomycin in HILIC, Cation-Exchange Modes on Amaze TCH Column
Conditions of Experiment
| Column: | Amaze TCH |
| Separation Modes: | HILIC and cation-exchange |
| Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
| Mobile Phase: | 75% ACN with 50 mM AmFm pH 2.7 |
| Detection: | ELSD |
| Sample: | 0.3 mg/ml |
| Injection: | 2 uL |
| Flow rate: | 0.6 ml/min |
Analytes
| Class of compounds: | Amines, Aminoglycoside, Antibiotic, Drug |
| Nature of compounds: | Basic, Hydrophilic |
| Compounds: | Dihydrostreptomycin, Streptomycin |