HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column
Conditions of Experiment
| Column: | Heritage MA |
| Separation Modes: | reversed-phase and anion-exchange |
| Column Dimenstions: | 4.6 x 150 mm |
| Mobile Phase: | 20% ACN with 100 mM AmFm pH 3 |
| Detection: | UV 255 nm |
| Sample: | 0.3 mg/ml |
| Injection: | 1 uL |
| Flow rate: | 1 mL/min |
Analytes
| Class of compounds: | Aromatic acid, Catecholamine, Neurotransmitter, Organic acid |
| Nature of compounds: | Acidic, Hydrophilic, Polar |
| Compounds: | Vanillylmandelic Acid, Mandelic acid, Atrolactic acid, α-Methoxyphenylacetic acid |